|
Status |
Public on Aug 19, 2009 |
Title |
*05-074 |
Sample type |
RNA |
|
|
Source name |
No fusion B-ALL
|
Organism |
Homo sapiens |
Characteristics |
tissue: Bone marrow ethnicity: Chinese disease: pediatric acute lymphoblastic leukemia chinese pediatric all subtype: No fusion B-ALL relapsed: yes
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cryopreserved mononuclear cell suspensions from BM samples using Trizol (Invitrogen) and purified with the RNeasy Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were synthesized with One-Cycle Target Labeling and Control Reagents (Affymetrix).
|
|
|
Hybridization protocol |
The labeled RNA was then fragmented and hybridized to HG-U133A 2.0 oligonucleotide arrays (Affymetrix Incorporated, Santa Clara, CA) according to Affymetrix protocols.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
Description |
Chinese ALL sample. Sample ID: 05-074
|
Data processing |
Data were processed by using the robust multi-array analysis (RMA) from the Bioconductor package.
|
|
|
Submission date |
Aug 18, 2009 |
Last update date |
Aug 25, 2009 |
Contact name |
Nan Qiao |
Organization name |
Institute of Genetics and Developmental Biology
|
Department |
System Biology
|
Street address |
Datun Road
|
City |
Beijing |
ZIP/Postal code |
100101 |
Country |
China |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE17703 |
Bone marrow gene expression of pediatric acute lymphoblastic leukemia (ALL) |
|