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Sample GSM4413300 Query DataSets for GSM4413300
Status Public on Mar 17, 2020
Title TissuePanel_FO916_Gonad
Sample type SRA
 
Source name Gonad
Organism Mus musculus
Characteristics cell type: Gonad
treatment: No dox
system: cCARLIN
Extracted molecule polyA RNA
Extraction protocol Tissues were dissected, snap frozen, and the RNA extracted using Trizol
RNA of up to 1ug or 150,000 cells was reverse transcribed using a CARLIN-specific UMI-tagged primer. The CARLIN array was then PCR amplified by nested PCR before indexing and sequencing via MiSeq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
 
Data processing Cellranger v2.2.0 (with default parameters) was used to process single-cell RNA sequencing datasets prepared using CellRanger V2 Chemistry
Cellranger v3.0.2 (with default parameters) was used to process single-cell RNA sequencing datasets prepared using CellRanger V3 Chemistry
Genome_build: mm10
Supplementary_files_format_and_content: H5 - CellRanger single-cell count matrix
 
Submission date Mar 16, 2020
Last update date Mar 17, 2020
Contact name Duluxan Sritharan
Organization name Harvard University
Street address 240 Longwood Avenue, Bldg. C
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL16417
Series (1)
GSE146972 An engineered CRISPR/Cas9 mouse line for simultaneous readout of lineage histories and gene expression profiles in single cells
Relations
BioSample SAMN14382789
SRA SRX7916469

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data not provided for this record

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