|
Status |
Public on Mar 17, 2020 |
Title |
Inducible_FO406_50K |
Sample type |
SRA |
|
|
Source name |
Bone marrow granulocytes
|
Organism |
Mus musculus |
Characteristics |
cell type: Bone marrow granulocytes treatment: Dox system: iCARLIN
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Granulocytes were sorted by FACS and resuspended in Trizol RNA of up to 1ug or 150,000 cells was reverse transcribed using a CARLIN-specific UMI-tagged primer. The CARLIN array was then PCR amplified before indexing and sequencing via MiSeq
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina MiSeq |
|
|
Data processing |
Cellranger v2.2.0 (with default parameters) was used to process single-cell RNA sequencing datasets prepared using CellRanger V2 Chemistry Cellranger v3.0.2 (with default parameters) was used to process single-cell RNA sequencing datasets prepared using CellRanger V3 Chemistry Genome_build: mm10 Supplementary_files_format_and_content: H5 - CellRanger single-cell count matrix
|
|
|
Submission date |
Mar 16, 2020 |
Last update date |
Mar 17, 2020 |
Contact name |
Duluxan Sritharan |
Organization name |
Harvard University
|
Street address |
240 Longwood Avenue, Bldg. C
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
|
|
Platform ID |
GPL16417 |
Series (1) |
GSE146972 |
An engineered CRISPR/Cas9 mouse line for simultaneous readout of lineage histories and gene expression profiles in single cells |
|
Relations |
BioSample |
SAMN14382817 |
SRA |
SRX7916347 |