|
| Status |
Public on Dec 01, 2011 |
| Title |
Ewing sarcoma patient sample R50 (set B) |
| Sample type |
RNA |
| |
|
| Source name |
fresh frozen tissue sample
|
| Organism |
Homo sapiens |
| Characteristics |
sample id: R50
diagnosis: PNET
state: Metastasis
age: 16
sex: M
efs (months): 58.3
ovs (months): 99.3
status: Dead
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Cell lines were cultured according to Manara et al., 2002
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Invitrogen TRIzol extraction kit according to manufacture's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix GeneChip One-Cycle Target Labelling protocol from 5 ug of total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned using GeneChip Scanner 3000
|
| Description |
n/a
|
| Data processing |
Images were analysed using GeneChip Operating Software
|
| |
|
| Submission date |
Aug 17, 2009 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Suvi Savola |
| URL |
http://www.helsinki.fi/cmg
|
| Organization name |
University of Helsinki
|
| Department |
Pathology
|
| Lab |
Laboratory of Cytomolecular Genetics
|
| Street address |
Haartmaninkatu 3
|
| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE17674 |
Inflammatory gene profiling of Ewing sarcoma family of tumors (set B) |
| GSE17679 |
Inflammatory gene profiling of Ewing sarcoma family of tumors |
|
