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Sample GSM441000 Query DataSets for GSM441000
Status Public on Dec 01, 2011
Title Ewing sarcoma patient sample R196 (set B)
Sample type RNA
 
Source name fresh frozen tissue sample
Organism Homo sapiens
Characteristics sample id: R196
diagnosis: Ewing
tissue: tumor
state: Primary
age: 5
sex: M
efs (months): 14
ovs (months): 20.7
status: Dead
Treatment protocol not applicable
Growth protocol Cell lines were cultured according to Manara et al., 2002
Extracted molecule total RNA
Extraction protocol RNA was extracted using Invitrogen TRIzol extraction kit according to manufacture's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix GeneChip One-Cycle Target Labelling protocol from 5 ug of total RNA.
 
Hybridization protocol Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Arrays were scanned using GeneChip Scanner 3000
Description n/a
Data processing Images were analysed using GeneChip Operating Software
 
Submission date Aug 17, 2009
Last update date Dec 01, 2011
Contact name Suvi Savola
URL http://www.helsinki.fi/cmg
Organization name University of Helsinki
Department Pathology
Lab Laboratory of Cytomolecular Genetics
Street address Haartmaninkatu 3
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platform ID GPL570
Series (2)
GSE17674 Inflammatory gene profiling of Ewing sarcoma family of tumors (set B)
GSE17679 Inflammatory gene profiling of Ewing sarcoma family of tumors

Data table header descriptions
ID_REF
VALUE RMA normalized signal

Data table
ID_REF VALUE
1007_s_at 8.256538459
1053_at 5.761278303
117_at 4.615231854
121_at 7.457692989
1255_g_at 3.063165827
1294_at 6.356030336
1316_at 6.125153338
1320_at 4.140436437
1405_i_at 5.415820925
1431_at 3.740283993
1438_at 5.512185299
1487_at 6.558549442
1494_f_at 4.767850007
1552256_a_at 6.900099323
1552257_a_at 6.632831743
1552258_at 4.309139097
1552261_at 4.370877367
1552263_at 4.125805462
1552264_a_at 4.6782507
1552266_at 3.472633365

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM441000.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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