|
Status |
Public on Dec 01, 2011 |
Title |
Ewing sarcoma patient sample R51 (set A) |
Sample type |
RNA |
|
|
Source name |
freshfrozen tissue sample
|
Organism |
Homo sapiens |
Characteristics |
sample id: R51 diagnosis: Askin state: Primary age: 24 sex: M efs (months): 4.6 ovs (months): 10.9 status: Dead
|
Treatment protocol |
not applicable
|
Growth protocol |
Cell lines were cultured according to Manara et al., 2002
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Invitrogen TRIzol extraction kit according to manufacture's instructions
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix GeneChip One-Cycle Target Labelling protocol from 5 ug of total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
Arrays were scanned using GeneChip Scanner 3000
|
Description |
n/a
|
Data processing |
Images were analysed using GeneChip Operating Software
|
|
|
Submission date |
Aug 12, 2009 |
Last update date |
Dec 01, 2011 |
Contact name |
Suvi Savola |
URL |
http://www.helsinki.fi/cmg
|
Organization name |
University of Helsinki
|
Department |
Pathology
|
Lab |
Laboratory of Cytomolecular Genetics
|
Street address |
Haartmaninkatu 3
|
City |
Helsinki |
ZIP/Postal code |
00014 |
Country |
Finland |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE17618 |
Inflammatory gene profiling of Ewing sarcoma family of tumors (set A) |
GSE17679 |
Inflammatory gene profiling of Ewing sarcoma family of tumors |
|