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Sample GSM4332644 Query DataSets for GSM4332644
Status Public on Mar 22, 2021
Title Human_H3K27ac_MAIT_2
Sample type SRA
Source name MAIT cells
Organism Homo sapiens
Characteristics tissue: peripheral blood
cell type: MAIT cells
group: Treatment
chip antibody: H3K27ac
Extracted molecule genomic DNA
Extraction protocol Chromatin was immunoprecipitated using the Active Motif Low Cell ChIP-seq kit (53084) using 100,000 cells. Chromatin was sheared using a Diagenode Bioruptor Pico (B01060002)
Libraries were generated using the Active Motif Low Cell ChIP-seq kit (53084).
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 3000
Description CD3+ CD161+ TCR Vα7.2+
Data processing Fastq files prepared using Illumina FASTQ Generation application
Fastq files were trimmed using Trimmomatic
Reads were aligned to the hg19 genome using Bowtie2
Duplicate reads were removed using PicardTools
Peaks were called using Macs2
High confidence peaks were determined using IDR with a threshold IDR < 0.05
Genome_build: hg19
Supplementary_files_format_and_content: Bedgraph files were generated from Macs2 output
Submission date Feb 23, 2020
Last update date Mar 22, 2021
Contact name Dorina Avram
Organization name Moffitt Cancer Center
Department Department of Immunology
Lab Avram lab
Street address 12902 Magnolia Drive, MRC4
City Tampa
State/province Fl
ZIP/Postal code 33612
Country USA
Platform ID GPL21290
Series (2)
GSE145777 Mapping H3K27ac in human MAIT cells
GSE145779 Bcl11b binding and transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection
BioSample SAMN14167553
SRA SRX7785334

Supplementary file Size Download File type/resource
GSM4332644_Human_H3K27ac_MAIT_2_vs_Input_subtract.bedgraph.gz 238.7 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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