|
Status |
Public on Mar 22, 2021 |
Title |
Human_H3K27ac_MAIT_1 |
Sample type |
SRA |
|
|
Source name |
MAIT cells
|
Organism |
Homo sapiens |
Characteristics |
tissue: peripheral blood cell type: MAIT cells group: Treatment chip antibody: H3K27ac
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Chromatin was immunoprecipitated using the Active Motif Low Cell ChIP-seq kit (53084) using 100,000 cells. Chromatin was sheared using a Diagenode Bioruptor Pico (B01060002) Libraries were generated using the Active Motif Low Cell ChIP-seq kit (53084).
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 3000 |
|
|
Description |
CD3+ CD161+ TCR Vα7.2+
|
Data processing |
Fastq files prepared using Illumina FASTQ Generation application Fastq files were trimmed using Trimmomatic Reads were aligned to the hg19 genome using Bowtie2 Duplicate reads were removed using PicardTools Peaks were called using Macs2 High confidence peaks were determined using IDR with a threshold IDR < 0.05 Genome_build: hg19 Supplementary_files_format_and_content: Bedgraph files were generated from Macs2 output
|
|
|
Submission date |
Feb 23, 2020 |
Last update date |
Mar 22, 2021 |
Contact name |
Dorina Avram |
E-mail(s) |
dorina.avram@moffitt.org
|
Organization name |
Moffitt Cancer Center
|
Department |
Department of Immunology
|
Lab |
Avram lab
|
Street address |
12902 Magnolia Drive, MRC4
|
City |
Tampa |
State/province |
Fl |
ZIP/Postal code |
33612 |
Country |
USA |
|
|
Platform ID |
GPL21290 |
Series (2) |
GSE145777 |
Mapping H3K27ac in human MAIT cells |
GSE145779 |
Bcl11b binding and transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection |
|
Relations |
BioSample |
SAMN14167550 |
SRA |
SRX7785333 |