|
| Status |
Public on Jul 22, 2020 |
| Title |
OGT KO BMDMs; Unstimulated (M0) [F51] |
| Sample type |
SRA |
| |
|
| Source name |
BMDMs
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
genotype/variation: OGT KO
age: 12 weeks
cell type: Bone marrow-derived macrophages
treatment: Unstimulated (M0)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by using the RNeasy Plus Mini Kit (QIAGEN).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
F51
|
| Data processing |
Sequencing reads were aligned against the mouse mm10 reference genome using the TopHat2 software version 2.1.0 with the default parameters.
Mapped reads were assigned to gene features and quantified using the HTSeq software version 0.9.1.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: *txt: Tab-delimited text files include HTSeq raw abundance values for each sample.
|
| |
|
| Submission date |
Feb 21, 2020 |
| Last update date |
Jul 22, 2020 |
| Contact name |
Xiaoyong Yang |
| E-mail(s) |
xiaoyong.yang@yale.edu
|
| Organization name |
Yale University
|
| Department |
Comparative Medicine
|
| Street address |
375 Congress Ave
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520-8016 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE145720 |
OGT suppresses macrophage proinflammatory activation |
|
| Relations |
| BioSample |
SAMN14162089 |
| SRA |
SRX7778302 |
