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Sample GSM4310374 Query DataSets for GSM4310374
Status Public on Sep 23, 2020
Title SMG-KO
Sample type SRA
 
Source name Primary Mouse Epithelial Progenitor Cells
Organism Mus musculus
Characteristics age: Adult (12wk)
tissue: Salivary Gland
genotype/variation: p63 KO mouse
Extracted molecule total RNA
Extraction protocol Whole mouse Salivary gland was dissociated into single cells and sujected to single-cell sequencing
Chromimum Single Cell 3' v2 (10x Genomics)
TruSeq Stranded RNA (illumina)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description scRNA-Sequencing
Data processing Illumina RTA 2.2.68 Basecaller
Cellranger 2.1.0 mkoutput with Bcl2Fastq 2.19.1
Cellranger 2.1.0 Count against mm10 genome to produce count matrix
Seurat 3.1.2 used for marker gene analysis and visualization
Genome_build: mm10
Supplementary_files_format_and_content: CSV File containing UMI corrected read counts
 
Submission date Feb 13, 2020
Last update date Sep 23, 2020
Contact name Jonathan E Bard
E-mail(s) jbard@buffalo.edu
Organization name University at Buffalo
Department Genomics and Bioinformatics Core
Street address 701 Ellicott Street
City Buffalo
State/province New York
ZIP/Postal code 14203
Country USA
 
Platform ID GPL19057
Series (2)
GSE145267 p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling [scRNA-seq]
GSE145268 p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling
Relations
BioSample SAMN14096856
SRA SRX7719804

Supplementary file Size Download File type/resource
GSM4310374_SMG-KO_filtMatrix.csv.gz 5.4 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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