|
Status |
Public on Dec 20, 2020 |
Title |
Ba/F3_Phf23-ChIP-1 |
Sample type |
SRA |
|
|
Source name |
Ba/F3 cells
|
Organism |
Mus musculus |
Characteristics |
tissue: Ba/F3 cells plasmids: (MSCV-IRES-mCherry)pMIC-mPhf23-flag replicates: Phf23_rep1 antibody: FLAG M2 agarose beads(Sigma, A2220)
|
Treatment protocol |
Ba/F3 cells were transducted with mCherry labelled and flag tagged mouse Phf23 (pMIC-mPhf23-flag) through retroviral infection. 48h after infection, the mCherry positive cells were sorted out by FACS AriaIII and cultured in vitro.
|
Growth protocol |
Ba/F3 cells were cultured in RPMI 1640 medium supplemented with 10% FBS, 1% Pen/Strep and 2ng/ml mouse IL3.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
The ChIP DNA was purified with Qiagen MinElute PCR Purification Kit ChIP DNA was prepared according to the protocol provided in Methods.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
ChIP-seq reads were aligned to the mm10 genome assembly using STAR_2.6.0a Bam files were transformed to bigwig files by Deeptools peaks were called using macs2 2.1.1 processed data files format and content: bigWig and peak files
|
|
|
Submission date |
Feb 12, 2020 |
Last update date |
Dec 20, 2020 |
Contact name |
Xaingyu Pan |
E-mail(s) |
pangxueyu233@outlook.com
|
Organization name |
Sichuan University
|
Department |
State Key Laboratory of Biotherapy, West China Hospital, Sihcuan University.
|
Lab |
Chen & Liu Lab
|
Street address |
No.17 Renming road section3
|
City |
Chengdu, Cihna |
State/province |
Sichuan |
ZIP/Postal code |
610041 |
Country |
China |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE145190 |
An epigenetic mechanism underlying chromosome 17p deletion-driven tumorigenesis |
|
Relations |
BioSample |
SAMN14088617 |
SRA |
SRX7713738 |