|
| Status |
Public on Aug 10, 2021 |
| Title |
Actinin-BirA* 3 |
| Sample type |
SRA |
| |
|
| Source name |
iPSC-CMs (cardiomyocytes)
|
| Organism |
Homo sapiens |
| Characteristics |
age: Day 25
background: PGP1
cell type: iPSC-CMs (cardiomyocytes)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were trizoled and routine RNA extraction protocol was followed
Illumina TruSeq Stranded Total RNA library preparation with Ribo-Zero depletion for rRNA
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Trimmed reads were aligned using STAR
HTSeq was utilized to convert files to raw counts
Counts were analyzed tith DESeq2
Genome_build: h38
Supplementary_files_format_and_content: STAR alignment .bam files were converted to .counts using HTSeq (.counts files provided)
|
| |
|
| Submission date |
Feb 05, 2020 |
| Last update date |
Aug 10, 2021 |
| Contact name |
Travis Hinson |
| Organization name |
JAX-GM
|
| Street address |
10 Discovery Dr
|
| City |
Farmington |
| State/province |
Connecticut |
| ZIP/Postal code |
06030 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE144806 |
RNA-Immunoprecipitation Sequencing of Actinin-BirA* vs. Control iPSC-CMs |
|
| Relations |
| BioSample |
SAMN14003303 |
| SRA |
SRX7680932 |
