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Sample GSM4285907 Query DataSets for GSM4285907
Status Public on Jan 31, 2021
Title GTG_140_11: SKM-1
Sample type SRA
Source name SKM-1
Organism Homo sapiens
Characteristics cell line: SKM-1
disease: acute myeloid leukemia
treatment: ABBV-744
dose: 200 nM
Treatment protocol Cells were treated with DMSO or indicated inhibitors for 24 hr
Growth protocol Cell Lines were cultured in RPMI-1640 + 10% FBS
Extracted molecule total RNA
Extraction protocol RNA was prepared by Qiazol extraction followed by RNeasy mini column (QIAGEN)
10 ug of total RNA was submitted to poly-A selection to remove ribosomal RNA and reverse transcribed to double-stranded cDNA. The cDNA was eblunt ended, adenylated, and ligated with Illumina indexes.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Data processing Illumina Casava1.8 software used for basecalling
Reads were aligned using the OmicSoft aligner and gene expression quantified based on UCSC gene model.
Differentially expressed genes between treatment conditions detected by DESeq2 with a statistical cut-off (FC>2, p<0.01)
Genome_build: B37
Submission date Jan 27, 2020
Last update date Jan 31, 2021
Contact name Xin Lu
Phone 847-937-7942
Organization name Abbvie
Department Oncology Discovery
Street address 1 North Waukegan Rd
City North Chicago
State/province IL
ZIP/Postal code 60064
Country USA
Platform ID GPL21290
Series (1)
GSE144326 Selective inhibition of the second bromodomain of BET family proteins results in robust antitumor activity in preclinical models of acute myeloid leukemia
BioSample SAMN13930173
SRA SRX7637637

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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