|
| Status |
Public on Jan 06, 2021 |
| Title |
KO liver at 8W, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Gdown1 KO liver at 8W
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: Gdown1 KO
age: 8 weeks
tissue: Liver
|
| Treatment protocol |
Untreated
|
| Growth protocol |
Mice were maintained under controlled environmental conditions under a 12-h light /dark cycle and allowed ad libitum access to water and standard laboratory diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using the RNeasy kit from Qiagen according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Three hundred ng of total RNA was processed for cRNA synthesis with the Ambion WT Expression Kit (PN4411974). Fragmentation and terminal labeling were performed with the Affymetrix GeneChip WT Terminal Labeling Kit (PN 900671).
|
| |
|
| Hybridization protocol |
Hybridization was performed at 45 degrees Celsius for 17 hours. The arrays were washed and stained in the GeneChip Fluidic Station 450 (Affymetrix) by using Hybridization, Wash and Stain kit (Affymetrix).
|
| Scan protocol |
The arrays were scanned by the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gdown1_KO1
Gene expression data from Gdown1 KO liver at 8W.
|
| Data processing |
The data expressed as CEL files were normalized by the robust multiarray average (RMA) method with the Expression Console software (Affymetrix).
|
| |
|
| Submission date |
Jan 24, 2020 |
| Last update date |
Jan 06, 2021 |
| Contact name |
Miki JIshage |
| E-mail(s) |
jishagm@mail.rockefeller.edu
|
| Phone |
212-327-7605
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE144209 |
Differentially expressed genes in Gdown1 KO liver [array] |
| GSE144212 |
Transcriptional down-regulation of metabolic genes by Gdown1 ablation induces quiescent cell re-entry into the cell cycle |
|
