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Sample GSM4282417 Query DataSets for GSM4282417
Status Public on Jan 27, 2021
Title Meninges, Young
Sample type SRA
Source name Dural meninges
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: Dural meninges
cell type: Endothelial and mural
age: 2 months
Sex: Male
Treatment protocol not applicable
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol 10x Genomics Chromium single cell preparation
NGS Illumina Next Seq
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description Dural endothelial (CD45-CD31+CD13-) and mural cells (CD34-CD13+CD31-) were sorted from 5 mice
Data processing Illumina bcl2fastq software used for basecalling
UMI count matrices generated using the Cellranger count pipeline. Fastq files from each run were specified using the --fastqs flag The cellranger count pipeline, resulted in one count matrix for each of our sample conditions (brain old, brain young, meninges old, meninges young)
Genome_build: mm10
Supplementary_files_format_and_content: tar archive containing the raw UMI count matrices for each sample as well as matrices with cell barcodes and gene symbols.
Submission date Jan 23, 2020
Last update date Jan 27, 2021
Contact name Jonathan Kipnis
Organization name Washington University in Saint Louis
Department Pathology and Immunology
Lab Kipnis Lab
Street address 4515 McKinley Ave
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platform ID GPL19057
Series (2)
GSE144174 Cellular and molecular characterization of the dural sinuses as a neuroimmune interface [vasculature]
GSE144175 Cellular and molecular characterization of the dural sinuses as a neuroimmune interface
BioSample SAMN13910675
SRA SRX7625311

Supplementary file Size Download File type/resource
GSM4282417_MY.tar.gz 3.3 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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