NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM4282408 Query DataSets for GSM4282408
Status Public on Jan 27, 2021
Title Myeloid cells, A488
Sample type SRA
 
Source name Dura
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: Dura
cell type: Myeloid cells
age: 2 months
Sex: Male
treatment: OVA-488 positive only
Treatment protocol Intracisternal and intravenous injections of Ovalbumin
Growth protocol Not applicable
Extracted molecule total RNA
Extraction protocol 10x Genomics Chromium single cell preparation
NGS Illumina Next Seq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description Dural myeloid cells were sorted from 20 mice
Data processing Illumina bcl2fastq software used for basecalling
UMI count matrices generated using the Cellranger count pipeline. Fastq files from each run were specified using the --fastqs flag The cellranger count pipeline, resulted in one count matrix for each of our sample conditions (A488 + (IV injection) A647+ (ICM injection, Double Positive, Double Negative)
Genome_build: mm10
Supplementary_files_format_and_content: tar archive containing the raw UMI count matrices for each sample as well as matrices with cell barcodes and gene symbols.
 
Submission date Jan 23, 2020
Last update date Jan 27, 2021
Contact name Jonathan Kipnis
Organization name Washington University in Saint Louis
Department Pathology and Immunology
Lab Kipnis Lab
Street address 4515 McKinley Ave
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL19057
Series (2)
GSE144172 Cellular and molecular characterization of the dural sinuses as a neuroimmune interface [Myeloid cells]
GSE144175 Cellular and molecular characterization of the dural sinuses as a neuroimmune interface
Relations
BioSample SAMN13910673
SRA SRX7625612

Supplementary file Size Download File type/resource
GSM4282408_A488.tar.gz 6.3 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap