|
| Status |
Public on Jan 06, 2021 |
| Title |
09-19-12_JCipollo5_SG_Celegans_5_bus2_D2 |
| Sample type |
RNA |
| |
|
| Source name |
JCipollo5
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: bus2
|
| Treatment protocol |
L2-L3 stage
|
| Growth protocol |
mRNA is converted to cDNA and amplified using NuGEN pico amplification kit
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were nomanized by RMA with Parteck Genomic Suit 6.6
|
| |
|
| Submission date |
Jan 14, 2020 |
| Last update date |
Jan 06, 2021 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE143664 |
Altered Innate Immunity Confers Staphylococcus aureus Resistance in O-glycosylation Deficient Caenorhabditis elegans bus Mutants |
|
