|
Status |
Public on Jan 06, 2021 |
Title |
09-19-12_JCipollo5_SG_Celegans_5_bus2_D2 |
Sample type |
RNA |
|
|
Source name |
JCipollo5
|
Organism |
Caenorhabditis elegans |
Characteristics |
strain: bus2
|
Treatment protocol |
L2-L3 stage
|
Growth protocol |
mRNA is converted to cDNA and amplified using NuGEN pico amplification kit
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Data processing |
The data were nomanized by RMA with Parteck Genomic Suit 6.6
|
|
|
Submission date |
Jan 14, 2020 |
Last update date |
Jan 06, 2021 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL200 |
Series (1) |
GSE143664 |
Altered Innate Immunity Confers Staphylococcus aureus Resistance in O-glycosylation Deficient Caenorhabditis elegans bus Mutants |
|