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Sample GSM4260287 Query DataSets for GSM4260287
Status Public on Jan 13, 2020
Title PCAGGS-3
Sample type SRA
 
Source name HEK 293T_PCAGGS
Organism Homo sapiens
Characteristics cell line: HEK 293T
cell type: Human embryo kidney cell line
genotype/variation: Transfection of empty backbone
Extracted molecule total RNA
Extraction protocol Using TRIzol based method following manufactures instruction
Using VAZYME NR612 following manufactures instruction
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Data processing FastQC
Hisat2
Samtools
StringTie
Deseq2
Genome_build: hg19
Supplementary_files_format_and_content: Normalized read count matrix processed by Deseq2
 
Submission date Jan 12, 2020
Last update date Jan 15, 2020
Contact name Zhou Shen
E-mail(s) szhou1314@webmail.hzau.edu.cn
Organization name Huazhong agriculture university
Street address 1 Shi-zi-shan Street
City Wuhan
State/province Hubei
ZIP/Postal code 430070
Country China
 
Platform ID GPL21290
Series (1)
GSE143485 nsp1 acts on HEK-293T
Relations
BioSample SAMN13830699
SRA SRX7544050

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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