|
| Status |
Public on Jan 13, 2020 |
| Title |
PCAGGS-2 |
| Sample type |
SRA |
| |
|
| Source name |
HEK 293T_PCAGGS
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK 293T
cell type: Human embryo kidney cell line
genotype/variation: Transfection of empty backbone
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using TRIzol based method following manufactures instruction
Using VAZYME NR612 following manufactures instruction
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
FastQC
Hisat2
Samtools
StringTie
Deseq2
Genome_build: hg19
Supplementary_files_format_and_content: Normalized read count matrix processed by Deseq2
|
| |
|
| Submission date |
Jan 12, 2020 |
| Last update date |
Jan 15, 2020 |
| Contact name |
Zhou Shen |
| E-mail(s) |
szhou1314@webmail.hzau.edu.cn
|
| Organization name |
Huazhong agriculture university
|
| Street address |
1 Shi-zi-shan Street
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN13830700 |
| SRA |
SRX7544049 |
