|
Status |
Public on Jan 13, 2020 |
Title |
PCAGGS-1 |
Sample type |
SRA |
|
|
Source name |
HEK 293T_PCAGGS
|
Organism |
Homo sapiens |
Characteristics |
cell line: HEK 293T cell type: Human embryo kidney cell line genotype/variation: Transfection of empty backbone
|
Extracted molecule |
total RNA |
Extraction protocol |
Using TRIzol based method following manufactures instruction Using VAZYME NR612 following manufactures instruction
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 3000 |
|
|
Data processing |
FastQC Hisat2 Samtools StringTie Deseq2 Genome_build: hg19 Supplementary_files_format_and_content: Normalized read count matrix processed by Deseq2
|
|
|
Submission date |
Jan 12, 2020 |
Last update date |
Jan 15, 2020 |
Contact name |
Zhou Shen |
E-mail(s) |
szhou1314@webmail.hzau.edu.cn
|
Organization name |
Huazhong agriculture university
|
Street address |
1 Shi-zi-shan Street
|
City |
Wuhan |
State/province |
Hubei |
ZIP/Postal code |
430070 |
Country |
China |
|
|
Platform ID |
GPL21290 |
Series (1) |
|
Relations |
BioSample |
SAMN13830701 |
SRA |
SRX7544048 |