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Sample GSM4251590 Query DataSets for GSM4251590
Status Public on Jan 01, 2022
Title 293T_PacBio_Sequencing
Sample type SRA
 
Source name cell line
Organism Homo sapiens
Characteristics cell type: 293T
passage: 15-18
Treatment protocol No treatment or Transfection
Growth protocol Cells were maintained in DMEM (high glucose) (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Hyclone), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco).
Extracted molecule total RNA
Extraction protocol RNA was extracted from cell lines using Trizol
Sequencing libraries were prepared using standard protocols. Samll RNA library was constructed by NEBNext® Multiplex Small RNA Library Prep Set according to the manufacturer's’ protocol
PacBio Sequencing or MiSeq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model PacBio RS II
 
Description wild type cells
Data processing PacBio sequencing was processed by the Iso-Seq standard pipeline.
Small RNA sequencing was processed by customized scripts. Reads without the 3′ adaptor sequences or shorter than 18 nt were discarded
Genome_build: GRCH38 or miRBase21
Supplementary_files_format_and_content: BED files or TXT files
 
Submission date Jan 06, 2020
Last update date Jan 01, 2022
Contact name Lisheng Dai
E-mail(s) lisheng.dai@nih.gov
Phone 3018466653
Organization name NCI-Frederick
Street address 1050 Boyles St.
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL21311
Series (1)
GSE143169 Novel, abundant Drosha isoforms are deficient in miRNA processing in cancer cells
Relations
BioSample SAMN13739593
SRA SRX7505751

Supplementary file Size Download File type/resource
GSM4251590_293T_isoseq_flnc.fasta.gz 5.6 Mb (ftp)(http) FASTA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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