|
| Status |
Public on Dec 05, 2021 |
| Title |
hESC O 3 |
| Sample type |
RNA |
| |
|
| Source name |
Control
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human Embryonic Stem Cells
gender: male
tissue: embryo
|
| Growth protocol |
hESC were grown on mitotically inactivated MEFs on 0,1% gelatin-coated plates and cultured in hESC media containing DMEM/F-12 +Glutamax (Gibco) supplemented with 20% Knockout Serum replacement (Gibco), 1% PenStrep (Gibco), 1% NEAA (Gibco) and 0,1mM beta-mercaptoethanol (Gibco). FGF2 (Peprotech) was added freshly to a final concentration of 10ng/ul
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Mini Kit (Qiagen) following the manufacturers' instructions. DNAseI treatment (Ambion DNA-free kit, Invitrogen) was performed according to manufacturers' instructions
|
| Label |
Biotin, Cy3-Streptavidin
|
| Label protocol |
The Illumina TotalPrep RNA Amplification Kit (life technologies) was used to generate single-stranded cRNA from input amounts of 200ng total RNA
|
| |
|
| Hybridization protocol |
Standard Illumina Hybridisation Protocol (Whole-Genome Gene Expression Direct Hybridization Assay Guide) 750 ng of cRNA were hybridized for 17 hr at 58°C on Illumina Human HT-12-Microarray.
|
| Scan protocol |
Standard Illumina Scanning Protocol (Whole-Genome Gene Expression Direct Hybridization Assay Guide) (Whole-Genome Gene Expression Direct Hybridization Assay Guide)
|
| Description |
200757770002_C
replicate 3
|
| Data processing |
data were normalised using quantile normalisation with R using function normalize.quantiles from Bioconductor package preprocessCore
|
| |
|
| Submission date |
Dec 18, 2019 |
| Last update date |
Dec 05, 2021 |
| Contact name |
Alicia Roig Merino |
| Organization name |
DKFZ
|
| Street address |
Im Neuenheimer Feld 242
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE142299 |
Analysis of gene expression of hESCs upon genetic modification with EBNA and S/MAR episomal vectors |
|
