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Status |
Public on Sep 08, 2020 |
Title |
Old_Tet2-/-_S1615 (whs v17) |
Sample type |
SRA |
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Source name |
oocyte
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Organism |
Mus musculus |
Characteristics |
strain: C57BL6/129 age: old(10-12M) genotype: Tet2 knock out cell type: single MII oocyte
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Extracted molecule |
total RNA |
Extraction protocol |
For oocyte sequence, we obtained 133 ovulated oocytes; for PGC sequence, we purified E13.5 PGC from 4-6 embryos each group oocyte library were prepared using standard Illumina protocols; PGC library were prepared according to micro RNA construction
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
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Description |
whs_v17.txt
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Data processing |
we analysed the raw data of fq.gz files by Hisat2,samtools and featurecounts to get the raw counts. Genome_build: mm10 Supplementary_files_format_and_content: The reads counts of 133 samples, containg young WT, young Tet2-/-, old WT and old Tet2-/-, and some of Tet1 samples.
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Submission date |
Dec 17, 2019 |
Last update date |
Sep 08, 2020 |
Contact name |
wang hua song |
E-mail(s) |
2120160958@mail.nankai.edu.cn
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Organization name |
nankai university
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Street address |
Tianjin city, China
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City |
Tianjin |
ZIP/Postal code |
300071 |
Country |
China |
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Platform ID |
GPL21273 |
Series (1) |
GSE142163 |
Oocyte RNA-seq data of WT, Tet1-/- and Tet2-/-; RNA seq of PGC at E13.5 |
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Relations |
BioSample |
SAMN13612317 |
SRA |
SRX7396503 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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