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Sample GSM4221797 Query DataSets for GSM4221797
Status Public on Sep 08, 2020
Title Old_Tet2-/-_S1612 (whs v17)
Sample type SRA
Source name oocyte
Organism Mus musculus
Characteristics strain: C57BL6/129
age: old(10-12M)
genotype: Tet2 knock out
cell type: single MII oocyte
Extracted molecule total RNA
Extraction protocol For oocyte sequence, we obtained 133 ovulated oocytes; for PGC sequence, we purified E13.5 PGC from 4-6 embryos each group
oocyte library were prepared using standard Illumina protocols; PGC library were prepared according to micro RNA construction
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
Description whs_v17.txt
Data processing we analysed the raw data of fq.gz files by Hisat2,samtools and featurecounts to get the raw counts.
Genome_build: mm10
Supplementary_files_format_and_content: The reads counts of 133 samples, containg young WT, young Tet2-/-, old WT and old Tet2-/-, and some of Tet1 samples.
Submission date Dec 17, 2019
Last update date Sep 08, 2020
Contact name wang hua song
Organization name nankai university
Street address Tianjin city, China
City Tianjin
ZIP/Postal code 300071
Country China
Platform ID GPL21273
Series (1)
GSE142163 Oocyte RNA-seq data of WT, Tet1-/- and Tet2-/-; RNA seq of PGC at E13.5
BioSample SAMN13612313
SRA SRX7396500

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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