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Sample GSM4217503 Query DataSets for GSM4217503
Status Public on Dec 05, 2021
Title SW480-exos-2
Sample type RNA
 
Source name exosomes from weakly metastatic CRC cells SW480-2
Organism Homo sapiens
Characteristics cell type: exosomes
cell line: SW480
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the mirVana miRNA Isolation Kit (Cat. AM1561, Invitrogen, Thermo Fisher Scientific Inc., USA) following the manufacturer’s protocol. RNA purity and quantification were evaluated using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
Label Cy3
Label protocol The sample labeling, microarray hybridization and washing were performed based on the manufacturer’s standard protocols. Briefly, total RNA were dephosphorylated, denatured and then labeled with Cyanine-3-CTP.
 
Hybridization protocol After purification the labeled RNAs were hybridized onto the microarray.
Scan protocol After washing, the arrays were scanned with the Agilent Scanner G2505C (Agilent Technologies).
Data processing Feature Extraction software (version10.7.1.1, Agilent Technologies) was used to analyze array images to get raw data. Next, GeneSpring software (version 14.8, Agilent Technologies) was employed to finish the basic analysis with the raw data. The raw data was normalized with the quantile algorithm.
 
Submission date Dec 13, 2019
Last update date Dec 05, 2021
Contact name Senlin Zhao
E-mail(s) sunshinezsl1989@hotmail.com
Phone 18017312693
Organization name Cancer Hospital, Fudan University
Department Colorectal Surgery
Street address 270 Dong An Road
City Shanghai
ZIP/Postal code 200032
Country China
 
Platform ID GPL24741
Series (1)
GSE141997 Tumor-derived exosomal miR-181a-5p activates hepatic stellate cells to promote liver metastasis of colorectal cancer

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
Blank -3.321928095
NC1_00000197 -3.321928095
NC1_00000215 -3.321928095
NC2_00079215 -3.321928095
NC2_00092197 -3.321928095
NC2_00106057 -3.321928095
NC2_00122731 -3.321928095
NegativeControl -3.321928095
dmr_285 10.24317867
dmr_3 11.0406399
dmr_308 -3.321928095
dmr_316 -3.321928095
dmr_31a 9.79065295
dmr_6 8.847587026
hsa-let-7a-3p -3.321928095
hsa-let-7a-5p 9.279245994
hsa-let-7b-3p 0.472363387
hsa-let-7b-5p 9.14705852
hsa-let-7c-3p -3.321928095
hsa-let-7c-5p 5.573679865

Total number of rows: 2570

Table truncated, full table size 69 Kbytes.




Supplementary file Size Download File type/resource
GSM4217503_S42.txt.gz 6.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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