|
Status |
Public on Apr 13, 2022 |
Title |
Trisomy 18 syndrome-2 |
Sample type |
SRA |
|
|
Source name |
human amniotic fluid cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: amniotic fluid cells
|
Extracted molecule |
total RNA |
Extraction protocol |
For scRNA-seq, the PBMC were dissociated into single cells suspensions using Ficoll-Paque buffer (Sigma), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements. Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information Genome_build: GRCh38 Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell
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|
|
Submission date |
Nov 26, 2019 |
Last update date |
Apr 13, 2022 |
Contact name |
Jing Wang |
E-mail(s) |
19011081019@stu.hqu.edu.cn
|
Phone |
18559219181
|
Organization name |
Huaqiao University
|
Department |
School of Medicine and School of Biomedical Sciences
|
Lab |
Center for Precision Medicine
|
Street address |
668 Jimei Road
|
City |
Xiamen |
State/province |
Fujian |
ZIP/Postal code |
361021 |
Country |
China |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE141074 |
Single-cell transcriptomics reveals diverse and complex gene expression alterations in human trisomy 18 |
|
Relations |
BioSample |
SAMN13391998 |
SRA |
SRX7223709 |