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Sample GSM4194532 Query DataSets for GSM4194532
Status Public on Apr 13, 2022
Title Normal-2
Sample type SRA
 
Source name human amniotic fluid cells
Organism Homo sapiens
Characteristics cell type: amniotic fluid cells
Extracted molecule total RNA
Extraction protocol For scRNA-seq, the PBMC were dissociated into single cells suspensions using Ficoll-Paque buffer (Sigma), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements.
Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell
 
Submission date Nov 26, 2019
Last update date Apr 13, 2022
Contact name Jing Wang
E-mail(s) 19011081019@stu.hqu.edu.cn
Phone 18559219181
Organization name Huaqiao University
Department School of Medicine and School of Biomedical Sciences
Lab Center for Precision Medicine
Street address 668 Jimei Road
City Xiamen
State/province Fujian
ZIP/Postal code 361021
Country China
 
Platform ID GPL24676
Series (1)
GSE141074 Single-cell transcriptomics reveals diverse and complex gene expression alterations in human trisomy 18
Relations
BioSample SAMN13392000
SRA SRX7223707

Supplementary file Size Download File type/resource
GSM4194532_W6-gene_cell_exprs_norm_table.xls.gz 223.4 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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