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Sample GSM4194532 Query DataSets for GSM4194532
Status Public on Apr 13, 2022
Title Normal-2
Sample type SRA
Source name human amniotic fluid cells
Organism Homo sapiens
Characteristics cell type: amniotic fluid cells
Extracted molecule total RNA
Extraction protocol For scRNA-seq, the PBMC were dissociated into single cells suspensions using Ficoll-Paque buffer (Sigma), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture’s protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing CellRanger was used to base-calling, alignment, filtering and generation of normalized abundance measurements.
Read 1: contains the 16bp 10x barcode and the 10bp UMI (bases 17-26).Index contains the sample barcode. Read 2 contains the cDNA information
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited text files include normalized median scaling values for each cell
Submission date Nov 26, 2019
Last update date Apr 13, 2022
Contact name Jing Wang
Phone 18559219181
Organization name Huaqiao University
Department School of Medicine and School of Biomedical Sciences
Lab Center for Precision Medicine
Street address 668 Jimei Road
City Xiamen
State/province Fujian
ZIP/Postal code 361021
Country China
Platform ID GPL24676
Series (1)
GSE141074 Single-cell transcriptomics reveals diverse and complex gene expression alterations in human trisomy 18
BioSample SAMN13392000
SRA SRX7223707

Supplementary file Size Download File type/resource
GSM4194532_W6-gene_cell_exprs_norm_table.xls.gz 223.4 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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