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Sample GSM4182448 Query DataSets for GSM4182448
Status Public on Feb 24, 2020
Title 9-Normal
Sample type RNA
Source name normal
Organism Homo sapiens
Characteristics tissue type: normal
sample type: Formalin-fixed paraffin-embedded blocks (FFPE Blocks)
Growth protocol tissue protocol: Formalin-fixed paraffin-embedded blocks of SPN specimens were obtained from the archives of the Institute of Pathology. Samples were reviewed, and ten micrometer thick sections were cut from the tumor block, and transferred on to glass slides. A single glass slide containing a block sample was stained with hematoxylin and eosin, and tumor margins were marked by the pathologist (EB). Microdissection was performed manually to extract tumoral tissue using a sharp scalpel.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using miRNeasy Formalin-fixed paraffin-embedded kit (Qiagen, Hilden, Germany), according to manufacturer’s instructions
Label biotin
Label protocol Genisphere FlashTag Biotin Labeling Assay according to the manufacturer's protocol
Hybridization protocol according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Scan protocol GenePix 4000B array scanner, according to the manufacturer's protocol
Data processing Gene-level analyses was performed. RMA normalization, summarization by mean, all were done by Partek Genomics suite (version 6.5) Copyright ©
Submission date Nov 20, 2019
Last update date Feb 25, 2020
Contact name Metsada Pasmanik-Chor
Organization name Tel Aviv University
Department Biology
Lab Bioinformatics Unit
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
Platform ID GPL19117
Series (1)
GSE140719 Micro-RNA Expression Patterns Predict Metastatic Spread in Solid Pseudopapillary Neoplasms of the Pancreas

Data table header descriptions
VALUE Log2 signal intensities after normalization and summarization

Data table
spike_in-control-2_st 13.5008
spike_in-control-23_st 13.3226
spike_in-control-31_st 12.4996
MIMAT0018976_st 11.9935
spike_in-control-36_st 11.2649
spike_in-control-29_st 10.896
gi555853_copy8_st 11.0778
gi555853_copy0_st 10.9451
gi555853_copy1_st 10.9871
gi555853_copy3_st 10.9004
gi555853_copy6_st 10.8938
gi555853_copy7_st 10.7872
MIMAT0000063_st 10.4618
gi555853_copy4_st 10.8103
gi555853_copy9_st 10.868
gi555853_copy5_st 10.579
gi555853_copy2_st 10.6209
MIMAT0022259_st 9.8213
MIMAT0019337_st 10.8023
MIMAT0000080_st 8.63824

Total number of rows: 6631

Table truncated, full table size 162 Kbytes.

Supplementary file Size Download File type/resource
GSM4182448_M497.cel.gz 667.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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