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Sample GSM4182444 Query DataSets for GSM4182444
Status Public on Feb 24, 2020
Title 3-LT-localized tumor
Sample type RNA
Source name Solid pseudopapillary neoplasm (SPN) of pancreas
Organism Homo sapiens
Characteristics tissue type: LT-localized tumor
sample type: Formalin-fixed paraffin-embedded blocks (FFPE Blocks)
Growth protocol tissue protocol: Formalin-fixed paraffin-embedded blocks of SPN specimens were obtained from the archives of the Institute of Pathology. Samples were reviewed, and ten micrometer thick sections were cut from the tumor block, and transferred on to glass slides. A single glass slide containing a block sample was stained with hematoxylin and eosin, and tumor margins were marked by the pathologist (EB). Microdissection was performed manually to extract tumoral tissue using a sharp scalpel.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using miRNeasy Formalin-fixed paraffin-embedded kit (Qiagen, Hilden, Germany), according to manufacturer’s instructions
Label biotin
Label protocol Genisphere FlashTag Biotin Labeling Assay according to the manufacturer's protocol
Hybridization protocol according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Scan protocol GenePix 4000B array scanner, according to the manufacturer's protocol
Data processing Gene-level analyses was performed. RMA normalization, summarization by mean, all were done by Partek Genomics suite (version 6.5) Copyright ©
Submission date Nov 20, 2019
Last update date Feb 25, 2020
Contact name Metsada Pasmanik-Chor
Organization name Tel Aviv University
Department Biology
Lab Bioinformatics Unit
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
Platform ID GPL19117
Series (1)
GSE140719 Micro-RNA Expression Patterns Predict Metastatic Spread in Solid Pseudopapillary Neoplasms of the Pancreas

Data table header descriptions
VALUE Log2 signal intensities after normalization and summarization

Data table
spike_in-control-2_st 13.5178
spike_in-control-23_st 13.3565
spike_in-control-31_st 12.5605
MIMAT0018976_st 12.2856
spike_in-control-36_st 11.5913
spike_in-control-29_st 11.1373
gi555853_copy8_st 10.739
gi555853_copy0_st 10.7638
gi555853_copy1_st 10.7648
gi555853_copy3_st 10.6342
gi555853_copy6_st 10.5735
gi555853_copy7_st 10.4555
MIMAT0000063_st 10.4596
gi555853_copy4_st 10.4708
gi555853_copy9_st 10.5695
gi555853_copy5_st 10.3342
gi555853_copy2_st 10.2983
MIMAT0022259_st 9.96056
MIMAT0019337_st 10.598
MIMAT0000080_st 9.71616

Total number of rows: 6631

Table truncated, full table size 162 Kbytes.

Supplementary file Size Download File type/resource
GSM4182444_M493.cel.gz 664.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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