tissue type: normal sample type: Formalin-fixed paraffin-embedded blocks (FFPE Blocks)
Growth protocol
tissue protocol: Formalin-fixed paraffin-embedded blocks of SPN specimens were obtained from the archives of the Institute of Pathology. Samples were reviewed, and ten micrometer thick sections were cut from the tumor block, and transferred on to glass slides. A single glass slide containing a block sample was stained with hematoxylin and eosin, and tumor margins were marked by the pathologist (EB). Microdissection was performed manually to extract tumoral tissue using a sharp scalpel.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using miRNeasy Formalin-fixed paraffin-embedded kit (Qiagen, Hilden, Germany), according to manufacturer’s instructions
Label
biotin
Label protocol
Genisphere FlashTag Biotin Labeling Assay according to the manufacturer's protocol
Hybridization protocol
according to the manufacturer's protocol (Affymetrix, Santa Clara, CA, USA)
Scan protocol
GenePix 4000B array scanner, according to the manufacturer's protocol
