|
| Status |
Public on Aug 02, 2013 |
| Title |
Before laughing vs After laughing prior to training in under-expression group |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Effective laugh maker training subjects
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral leukocyte
training: Effective laugh maker training
|
| Treatment protocol |
Training subjects were laughed according to "Effective laugh maker" instruction for one week. Control subjects spent one week without laughter instructed by "Effective laugh maker".
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from whole blood using the PAXgene™ Blood RNA System Kit following manufacturer's guidelines.
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
|
| |
|
| Channel 2 |
| Source name |
Effective laugh maker training subjects
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral leukocyte
training: Effective laugh maker training
|
| Treatment protocol |
Training subjects were laughed according to "Effective laugh maker" instruction for one week. Control subjects spent one week without laughter instructed by "Effective laugh maker".
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from whole blood using the PAXgene™ Blood RNA System Kit following manufacturer's guidelines.
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hybridization and wash protocol.
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 9.5.3.
|
| Description |
Training subjects were laughed according to "Effective laugh maker" instruction for one week.
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Jun 16, 2009 |
| Last update date |
Aug 02, 2013 |
| Contact name |
Miyo Hori |
| E-mail(s) |
hayashi@dna-chip.co.jp
|
| Phone |
81-45-500-5220
|
| Fax |
81-45-500-5219
|
| Organization name |
DNA Chip Research Inc.
|
| Department |
Business Development
|
| Street address |
1-1-43, Suehiro-cho
|
| City |
Tsurumi-ku, Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE16641 |
Laughter specific genes and laughter sensitive genes |
|
