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Status |
Public on Aug 26, 2010 |
Title |
The effect of ERG knockdown by shRNA in VCAP cells, array 1 |
Sample type |
RNA |
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Source name |
VCAP with ERG lenti-shRNA
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Organism |
Homo sapiens |
Characteristics |
cell line: VCAP transfection: ERG shRNA
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Treatment protocol |
VCAP cells were transfected with ERG lenti-shRNA and puromycin selection performed (2mg/ml). Thereafter, the total cellular RNAs were isolated by Trizol (Invitrogen).
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Growth protocol |
VCAP cells were grown in normal RPMI media.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol method from Invitrogen.
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Label |
biotin
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Label protocol |
cRNA was biotinylated during in vitro transcription reaction overnight (14h).
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Hybridization protocol |
1.5 µg each cRNA sample was hybridized to Illumina’s Bead Chip arrays at 58 °C overnight (19 h) according to Illumina 11225598_WGGEX_IntelliHyb_Seal_SystemManual_RevA.pdf.
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Scan protocol |
Chips were scanned with Illumina Bead Array Reader (Factor=1, PMT=521, Filter=100%)
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Description |
Human prostate cancer cells. VCAP cells were transfected with ERG lenti-shRNA and puromycin seletection performed ( 2mg/ml).Thereafter, the total cellular RNAs were isolated by trizol (Invitrogen).
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Data processing |
The numerical results were extracted with Bead Studio v3.2.6 without any normalization or background subtraction. Data were VST quantile normalized. Annotation: Illumina HumanRef-8 V3
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Submission date |
Jun 12, 2009 |
Last update date |
Apr 07, 2011 |
Contact name |
Santosh Kumar Gupta |
E-mail(s) |
79santosh@gmail.com
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Organization name |
VTT medical biotechnology
|
Department |
University of Turku
|
Lab |
VTT Medical Biotechnology (Olli Kallioniemi)
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Street address |
4th floor pharma city
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City |
Turku |
ZIP/Postal code |
20520 |
Country |
Finland |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE16671 |
The effect of ERG overexpression and ERG knockdown by shRNA in RWPE1 and VCAP cells, respectively |
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