RNA was extracted using the Qiagen Paxgene Blood RNA kit. Hemoglobin reduction was not performed.
Label
biotin
Label protocol
RNA was amplified using the Illumina TotalPrep RNA amplification kit, resulting in biotin labeled cRNA.
Hybridization protocol
Standard Illumina hybridizaion protocol
Scan protocol
Standard Illumina scanning protocol
Description
Control
Data processing
Robust multiarray analysis normalization was conducted in GeneSpring, resulting in background correction, quantile normalization, and summarization in the log base 2 scale.
