|
| Status |
Public on Nov 29, 2019 |
| Title |
CMV_plasmid_0_p-c_GEN00113848 |
| Sample type |
SRA |
| |
|
| Source name |
DNA plasmid
|
| Organism |
Adeno-associated virus |
| Characteristics |
experiment: capsid packaging screen
tissue: AAV genome DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
AAV were isolated according to methods described in paper
libraries were prepared using illumina sequencing primers
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
sequencing of AAV genome to determine packaing effeciency
|
| Data processing |
Library strategy: amplicon seq
for all samples, we use python to process fastqs and counted the barcodes
the counted barcodes are then merged onto a table containing the allele information for each barcode using python scripts
Genome_build: AAV2
Supplementary_files_format_and_content: barcode abundances and associated mutation information
|
| |
|
| Submission date |
Oct 31, 2019 |
| Last update date |
Nov 29, 2019 |
| Contact name |
Pierce Ogden |
| E-mail(s) |
pierceogden@gmail.com
|
| Organization name |
Harvard
|
| Lab |
Church Lab
|
| Street address |
3 Blackfan Circle, CLSB 528/4C
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL27708 |
| Series (1) |
| GSE139657 |
Comprehensive AAV capsid fitness landscape reveals a viral gene and enables machine-guided design |
|
| Relations |
| BioSample |
SAMN13168869 |
| SRA |
SRX7080445 |
