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Sample GSM4145060 Query DataSets for GSM4145060
Status Public on Oct 21, 2020
Title AML cells, Trib1 hi 2
Sample type RNA
 
Source name AML cell line expressing Hoxa9 and Trib1
Organism Mus musculus
Characteristics cell line: AML cell line
genotype: Hoxa9 and Trib1
Treatment protocol No treatment was provided.
Growth protocol Trib1 hi and null cells were grown in IMDM supplemented with 10% fetal bovine serum, and 5 ng/ml IL-3. Mouse AML cells developed in the Trib1 homozygous knock-out mouse.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
Description Gene expression data from mouse AML
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 31, 2019
Last update date Oct 21, 2020
Contact name Takuro Nakamura
E-mail(s) takuro-ind@umin.net
Phone 81-3-3570-0462
Organization name Japanese Foundation for Cancer Research
Department The Cancer Institute
Lab Carcinogenesis
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
 
Platform ID GPL11180
Series (2)
GSE139641 Trib1 promotes acute myeloid leukemia progression by modulating transcriptional programs of Hoxa9 [array]
GSE140315 Trib1 promotes acute myeloid leukemia progression by modulating transcriptional programs of Hoxa9

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 274.11588
AFFX-BioB-M_at 409.82895
AFFX-BioB-3_at 286.5374
AFFX-BioC-5_at 894.10236
AFFX-BioC-3_at 1140.7487
AFFX-BioDn-5_at 2397.8574
AFFX-BioDn-3_at 4213.907
AFFX-CreX-5_at 10780.639
AFFX-CreX-3_at 11807.195
AFFX-DapX-5_at 210.03004
AFFX-DapX-M_at 1064.5497
AFFX-DapX-3_at 1576.8202
AFFX-LysX-5_at 3.504158
AFFX-LysX-M_at 4.8411584
AFFX-LysX-3_at 6.026417
AFFX-PheX-5_at 6.8332043
AFFX-PheX-M_at 7.6922398
AFFX-PheX-3_at 8.260695
AFFX-ThrX-5_at 5.832979
AFFX-ThrX-M_at 5.8894343

Total number of rows: 45141

Table truncated, full table size 1057 Kbytes.




Supplementary file Size Download File type/resource
GSM4145060_hi1-TKO-H+T_2.ga.cel.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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