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Sample GSM4121570 Query DataSets for GSM4121570
Status Public on Oct 16, 2019
Title UnDiff_1
Sample type SRA
 
Source name Cell Line
Organism Homo sapiens
Characteristics cell line: BLaER1
Stage: Un-differentiaed
Treatment protocol BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/ml hrIL-3, hr-CSF-1 (M-CSF) (both MPI of Biochemistry, Munich) and 100 nM b-Estradiol.
Growth protocol BLaER1 cells were cultured in RPMI Medium 1640, supplemented with 10% (v/v) FCS, L-glutamine and 100 U/ml Penicillin-Streptomycin.
Extracted molecule total RNA
Extraction protocol Undifferentiated as well as differentiated BLaER1 cells were lysed in Trizol (2x106/1ml) and total RNA was purified using the Zymo Direct-zol RNA Miniprep.
Total RNA was used to generate stranded RNA sequencing libraries using the Encore Complete RNA-Seq™ library system of NuGEN.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Data processing Sequenced reads were trimmed for adaptor sequence with trimmomatic and aligned with STAR softare
The TPM (Transcripts Per Million) was quantified with RSEM
Genome_build: Ensembl release 91
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each sample
 
Submission date Oct 15, 2019
Last update date Oct 16, 2019
Contact name Veit Hornung
E-mail(s) hornung@genzentrum.lmu.de
Organization name LMU
Street address Feodor-Lynen-Straße 25
City Munich
ZIP/Postal code 81377
Country Germany
 
Platform ID GPL18460
Series (1)
GSE138913 RNASeq of differentiated and undifferentiated BLaER1 cells
Relations
BioSample SAMN13035630
SRA SRX6999916

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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