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Sample GSM41014 Query DataSets for GSM41014
Status Public on May 13, 2005
Title left ventricular myocardium, non-failing 5
Sample type RNA
Source name myocardium, left ventricular free wall
Organism Homo sapiens
Extracted molecule total RNA
Description Right atrial appendages were obtained from 30 patients undergoing open heart surgery for valve repair or coronary artery bypass grafting. Of these, ten patients had pmAF defined as duration of AF longer than three months as documented by ECG, and 20 patients had no history of AF and were in SR when open heart surgery was performed. Transmural LV tissue was obtained from five non-failing donor hearts which were not transplanted due to palpable coronary calcifications. After excision, all tissue specimens were immediately frozen in liquid nitrogen and stored at -80°C. Total RNA was purified from homogenized deep-frozen tissue samples following the TRIZOL standard protocol as described by the manufacturer (GibcoBRL, Eggenstein, Germany) and quantified by photometry. 150ng total RNA was used for quality control using the RNA 6000 Nano LabChip kit and Agilent Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA). Synthesis of double-stranded cDNA and of biotinylated cRNA probes as well as subsequent steps of hybridization, washing, and staining followed instructions provided by Affymetrix (Affymetrix, Santa Clara, CA, USA). One set of U133A+B chips (together representing 44.928 probe sets) was used for each human heart sample. All U133 GeneChips passed quality control to eliminate scans with abnormal characteristics, i.e. abnormal low or high dynamic range, high perfect match saturation, high pixel noise, grid misalignment problems, and low mean signal to noise ratio. Low level and statistical analysis was done using R 1.8.1. Microarray data of all atrial and ventricular samples was normalized simultaneously using robust multi-array average (RMA) implemented in Bioconductor's "affy"-package.
Keywords = Homo sapiens, myocardial tissue, comparison of right atrial tissue of patients with atrial fibrillation and sinus rhythm with left ventricular gene expression
Submission date Feb 03, 2005
Last update date Oct 28, 2005
Contact name Andreas S. Barth
Phone 0049-(0)89-7095-0
Organization name University of Munich (LMU), Grosshadern
Department Department of Cardiology
Street address Marchioninistr. 15
City Munich
ZIP/Postal code 81377
Country Germany
Platform ID GPL96
Series (1)
GSE2240 Comparison of atrial tissue of patients with atrial fibrillation and sinus rhythm with ventricular gene expression

Data table header descriptions
ID_REF Affymetrix probe set ID
VALUE 'signal' a measure of the abundance of a transcript (after normalization with robust-multiarray average (RMA) implemented in Bioconductor's affy-package)
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M).
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call, as calculated by the Bioconductor's affy-package.

Data table
1007_s_at 9.25808764 P 0.00868917
1053_at 6.633106685 P 0.027859596
117_at 7.618818286 P 0.017000453
121_at 9.889731567 P 0.011447358
1255_g_at 5.50630553 A 0.250723677
1294_at 8.205718601 P 0.031335632
1316_at 6.914291665 P 0.011447358
1320_at 7.424349558 A 0.284747112
1405_i_at 6.273762598 A 0.916687153
1431_at 5.628429974 A 0.250723677
1438_at 7.705127244 A 0.541184238
1487_at 9.59935698 P 0.004180367
1494_f_at 7.998500113 A 0.117160552
1598_g_at 12.11617449 P 0.000321571
160020_at 8.962069727 A 0.127645309
1729_at 8.474081567 A 0.541184238
177_at 6.737250182 A 0.189687302
1773_at 7.377178334 A 0.150527117
179_at 10.77768239 P 0.031335632
1861_at 7.337613755 P 0.027859596

Total number of rows: 22283

Table truncated, full table size 797 Kbytes.

Supplementary file Size Download File type/resource
GSM41014.CEL.gz 3.4 Mb (ftp)(http) CEL

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