|
| Status |
Public on Sep 17, 2022 |
| Title |
KO-2 |
| Sample type |
SRA |
| |
|
| Source name |
Islet
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 7 weeks
genotype: Sel1L knockout
|
| Growth protocol |
Following isolation, islets were recovered in 11mM RPMI overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
To dissociate islets into single cells, islets were incubated in trypsin for 15 minutes at 37 degree celcius.
RNA libraries were prepared for sequencing using standard 10X genomics protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Cell ranger (v.3.0.0) was used to QC and generate filtered gene matrices
Single-cell RNA seq data was processed by Seurat package (v.2.3.4).
Genome_build: mm10
Supplementary_files_format_and_content: Processed data files contain UMI count matrices from single cell RNA-seq.
|
| |
|
| Submission date |
Sep 20, 2019 |
| Last update date |
Sep 17, 2022 |
| Contact name |
Neha Shrestha |
| E-mail(s) |
nsstha@med.umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1000 Wall Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48195 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE137785 |
Sel1L-Hrd1 ERAD is required for the maintainenance of beta cell identity and function |
|
| Relations |
| BioSample |
SAMN12798009 |
| SRA |
SRX6879896 |
