|Public on Sep 19, 2022
|CAL-62_400 nM THZ531 rep2
|CAL-62_400 nM THZ531
|cell line: CAL-62
cell type: representative anaplastic thyroid carcinoma cell line
treatment: 400 nM THZ531 for 6h
|CAL-62 cells were treated in duplicate with either DMSO or THZ531 for 6 h.
|CAL-62 cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS).
|Total RNA was collected using TRIzol reagent following the manufacturer’s protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|Illumina NovaSeq 6000
|Raw data (raw reads) of fastq format were firstly processed through in-house perl scripts.
Clean reads were mapped to reference using Bowtie2. Only unique mapped reads were used in further analysis.
Differentially expressed genes were identified using DESeq2 algorithms.
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample.
|Sep 19, 2019
|Last update date
|Sep 19, 2022
|Tianjin Medical Universuty
|No.22 Qixiangtai Road
|Targeting CDK12-mediated transcription regulation in anaplastic thyroid carcinoma [RNA-seq]
|Targeting CDK12-mediated transcription regulation in anaplastic thyroid carcinoma