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Sample GSM4081554 Query DataSets for GSM4081554
Status Public on May 26, 2020
Title wt_eco_pc2
Sample type SRA
 
Source name peritoneal cavity neutrophil
Organism Mus musculus
Characteristics strain/background: C57BL/6
cell type: Peritoneal cavity neutrophil
condition: E. coli challenge (24h)
sorting: Sorted by Gr1+
Extracted molecule total RNA
Extraction protocol 10x Genomics Single Cell 3’ Library Kit V2.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description Single cell RNA-seq
Data processing Sequenced reads of mouse were aligned to the mm10 mouse transcriptome, then quantify the expression of transcripts in each cell using Cell Ranger v2.2.0.
Sequenced reads of human were aligned to the hg38 human transcriptome, then quantify the expression of transcripts in each cell using BD™ Rhapsody Whole Transcriptome Assay Analysis Pipeline.
Genome_build: mm10 (mouse) or hg38 (human)
Supplementary_files_format_and_content: *.txt: Text files include UMIs for each Sample.
 
Submission date Sep 16, 2019
Last update date May 27, 2020
Contact name Xuemei Xie
E-mail(s) xiexuemei.fiona@gmail.com
Organization name Boston Children's Hospital
Department Transfusion Medicine
Lab Luo's Lab
Street address 811 Enders Building, 320 Longwood Ave.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL24247
Series (2)
GSE137539 Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection
GSE137540 Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection
Relations
BioSample SAMN12769546
SRA SRX6855657

Supplementary file Size Download File type/resource
GSM4081554_wt_eco_pc2.txt.gz 12.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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