|
| Status |
Public on Jun 22, 2021 |
| Title |
TRAP 7-10h Input lms replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila embryos from stage 7-10h
|
| Organism |
Drosophila melanogaster |
| Characteristics |
origin: Staged collection
age: 7-10h AEL
muscle population: lms
|
| Growth protocol |
Embryos are collected from Drosophila fly population cages grown at 25 degres for 3 hours. Plates are then collected and kept at 25 degres for 7, 10 or 13 more hours to reach desired time windows
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After collection, embryos are washed and lysed in order to extract and immunoprecipitate GFP-tagged polysomes and associated RNA. Trizol extraction is then performed to purify RNA
|
| Label |
CY3
|
| Label protocol |
Labelling (Low Input Quick Amp WT Labeling Kit, One-Color), hybridization and scan were done at the Genecore genomics facility at EMBL Heidelberg using agilent 8X60K arrays technology (Agilent p/n G2534-60014) starting with 10ng of purified RNA.
|
| |
|
| Hybridization protocol |
Agilent Feature Extraction Software (v 10.7.3) was used for background subtraction and LOWESS normalization.
|
| Scan protocol |
Standard Agilent protocol
|
| Data processing |
signal intensities were normalized using quantile-quantile normalization with solo2 software. Log2 fold change were computed and p-value computed using t-test
|
| |
|
| Submission date |
Sep 13, 2019 |
| Last update date |
Jun 22, 2021 |
| Contact name |
yoan renaud |
| E-mail(s) |
yoan.renaud@uca.fr
|
| Organization name |
igred
|
| Street address |
place henri dunand
|
| City |
clermont ferrand |
| ZIP/Postal code |
63000 |
| Country |
France |
| |
|
| Platform ID |
GPL27460 |
| Series (1) |
| GSE137443 |
TRAP analysis of somatic muscle populations in Drosophila embryos |
|
