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Sample GSM4074036 Query DataSets for GSM4074036
Status Public on Jul 02, 2021
Title MYC_HMEC_TAM_SHY2_rep1 [RNA-seq]
Sample type SRA
 
Source name MYC-ER HMEC
Organism Homo sapiens
Characteristics cell line: MYC-ER HMEC
treatment: 4-Hydroxytamoxifen
cell culture: Medium 171/ MEGS supplement
Treatment protocol 4-Hydroxytamoxifen at 15nM for 48 hours
Growth protocol Standard
Extracted molecule total RNA
Extraction protocol Total RNA extraction using Trizol and Zymo's Directzol RNA extraction kit.
1ug total RNA was rRNA depleted and processed using the TruSeq Stranded Total RNA Preparation Kit by Illumina according to manufacturer’s instructions.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description featurecounts.txt
Data processing Raw sequencing reads were trimmed for adapter sequences using cutadapt. Trimmed reads were mapped against RepBase to remove reads mapping to repetitive sequences. Remaining reads were mapped to the appropriate genome build using STAR aligner.
Genome_build: hg19
Supplementary_files_format_and_content: Featurecounts
 
Submission date Sep 11, 2019
Last update date Jul 02, 2021
Contact name Gene Yeo
E-mail(s) geneyeo@ucsd.edu
Organization name UCSD
Street address 2880 Torrey Pines Scenic Dr. Room 3805/Yeo Lab
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL20301
Series (1)
GSE137258 Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer
Relations
BioSample SAMN12727113
SRA SRX6830241

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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