|Public on Jul 02, 2021
|cell line: MYC-ER HMEC
cell culture: Medium 171/ MEGS supplement
|4-Hydroxytamoxifen at 15nM for 48 hours
|250 ug RNA was phenol-chloroform extracted, rRNA depleted, and fragmented to ~100 nt. 1 ug fragmented RNA was saved as input and the rest was immunoprecipitated. RNA was precipitated and libraries were prepared with TruSeq Stranded Total RNA Preparation Kit according to manufacturer’s instructions.
|Illumina HiSeq 4000
|Library strategy: m6A-seq
Raw sequencing reads were trimmed for adapter sequences using cutadapt. Trimmed reads were mapped against RepBase to remove reads mapping to repetitive sequences. Remaining reads were mapped to the appropriate genome build using STAR aligner. Read densities were calculated to identify m6A peaks using MACS.
Supplementary_files_format_and_content: input normalized and filtered peaks in BED format and Peaks files (bigwigs).
|Sep 11, 2019
|Last update date
|Jul 03, 2021
|2880 Torrey Pines Scenic Dr. Room 3805/Yeo Lab
|Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer