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Sample GSM4073977 Query DataSets for GSM4073977
Status Public on Jul 02, 2021
Title T0_rep2_CRISPR_RBP
Sample type SRA
Source name MYC-ER HMEC
Organism Homo sapiens
Characteristics cell line: MYC-ER HMEC
treatment: None
timepoint: Day 0
cell culture: Medium 171/ MEGS supplement
Growth protocol Standard
Extracted molecule genomic DNA
Extraction protocol gDNA was extracted and sonicated to ~1000bp. sgRNA containing fragments were recovered with biotinylated RNA probes targeting the flanking region on the lenticrispr v2 backbone and pulled down with streptavidin beads. gDNA fragments were PCRed first with primers flanking the sgRNA and second with Illumina sequencing adapters.
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina HiSeq 4000
Description all.countsummary.txt
Data processing Library strategy: DNA-seq
CRISPR screening data was aligned and processed using MaGeCK.
Genome_build: hg19
Supplementary_files_format_and_content: MaGeCK processed gene summary
Submission date Sep 11, 2019
Last update date Jul 02, 2021
Contact name Gene Yeo
Organization name UCSD
Street address 2880 Torrey Pines Scenic Dr. Room 3805/Yeo Lab
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platform ID GPL20301
Series (1)
GSE137258 Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer
BioSample SAMN12727106
SRA SRX6830190

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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