|
| Status |
Public on Sep 09, 2020 |
| Title |
Normal Adipose, ctrl fat M2 |
| Sample type |
SRA |
| |
|
| Source name |
Normal Adipose
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: SB Transposase; onc3
phenotype: Normal Adipose
tissue: adipose
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lesions were disected, transferred to microcentrifuge tubes, snap frozen immediately in liquid nitrogen, and stored at -80C until RNA extraction. QIAzol was was used to lyse tissues, which were then homogenized. Qiagen RNeasy Lipid Tissue kit was used to isolate total RNA.
Illumina TruSeq unstranded poly-A selection kit (Cat# RS-122-2001) was used to make library from 1ug of RNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
IL15
|
| Data processing |
Fastq files were aligned to mm10 genome using STAR version 20201
Read counts per gene was quantified by STAR using Ensembl GRCm38.88 gtf file
Read counts normalized by voom were calculated using R package Limma.
Genome_build: mm10
Supplementary_files_format_and_content: Excel file reporting voom normalized log cpm
|
| |
|
| Submission date |
Sep 09, 2019 |
| Last update date |
Sep 09, 2020 |
| Contact name |
Safiyyah Ziyad |
| E-mail(s) |
sziyad@ucla.edu
|
| Organization name |
University of California, Los Angeles
|
| Department |
Molecular, Cell and Developmental Biology
|
| Lab |
Dr Luisa Iruela-Arispe
|
| Street address |
621 Charles E. Young Drive, South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL21493 |
| Series (2) |
| GSE137095 |
Endothelial mutagenesis screen identifies genetic risk factors in the development vascular anomalies [adipose] |
| GSE137097 |
Endothelial mutagenesis screen identifies genetic risk factors in the development vascular anomalies |
|
| Relations |
| BioSample |
SAMN12715354 |
| SRA |
SRX6818216 |
