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Sample GSM4066691 Query DataSets for GSM4066691
Status Public on Sep 01, 2021
Title 34_11_D5_1
Sample type SRA
Source name cardiac progenitor cells
Organism Homo sapiens
Characteristics cell type: ES-derived cardiac progenitor cells
passages: 8-13
Treatment protocol For cardiomyocyte differentiation, cells were induced using a chemically defined medium consisting of three components (CDM3): the basal medium RPMI 1640 (C14065500, Gibco), L-ascorbic acid 2-phosphate (213µg/ml,113170-55-1,Sigma) and Oryza sativa-derived recombinant human albumin (500µg/m,HY100M1,Healthgen Biotechnology Corp). In brief, single cell suspensions were prepared using Accutase and were seeded in 12-well Matrigel-coated plate at a density of 4×105 cells/well. When cells reached 80%-90% confluence (Day 0), cells were fed daily by 2ml CDM3 basal medium supplemented with CHIR99021 (6μM, 72052, Stem cell). 48 hours later (Day 2), medium was replaced with 2ml CDM3 supplemented with Wnt-C59 (2μM, 1248913, Peprotech Biogems). After 96 houres(day 4) , the medium was replaced with CDM3 basal medium (every other day) without any medium change until the appearance of cell beating.
Growth protocol Undifferentiated H1 hESC lines were maintained in a feeder-free culture system. Briefly, we precoated the well plates with Matrigel (354277, BD Biosciences), and then seeded and cultured cells with TeSR™-E8™ medium (05840,Stemcell). When cells reached 80% confluence, they were passaged routinely with Accutase (07920, Stemcell).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from D0, D2, , D3, D5 and D10 cells using Trizol reagent (15596018,Thermo Fisher Scientific). Reverse transcription was accomplished with Reverse Transcription Kit (RR037A,Takara) according to the manufacturer's instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing clean data were mapped to human reference genome hg19 by STAR_2.5.0a
HTSeq count was used for FPKM estimation
Differential expressed genes were identified by DESeq2
Genome_build: hg19
Supplementary_files_format_and_content: .txt file including fpkm
Submission date Sep 09, 2019
Last update date Sep 01, 2021
Contact name Fei Liang
Organization name Shanghai Jiao Tong University School of Medicine
Department Shanghai Children's Medical Center
Lab Zhen Zhang
Street address 1678 Dong Fang Road
City Shanghai
State/province Shanghai
ZIP/Postal code 200127
Country China
Platform ID GPL24676
Series (1)
GSE137090 RNA-Sequencing analysis of control and SORBS2 knockdown cardiac progenitor cells derived from human stem cells in vitro
BioSample SAMN12715036
SRA SRX6818044

Supplementary file Size Download File type/resource
GSM4066691_34_11_D5_1.fpkm.txt.gz 282.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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