|
| Status |
Public on Sep 04, 2019 |
| Title |
V08-EGM2 |
| Sample type |
RNA |
| |
|
| Source name |
EGM2 ASCs from subcutaneous adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell type: adipose-derived stem/stromal cells
growth: EGM2
|
| Treatment protocol |
Cells were resuspended by trypsination and were lysed with RLT (Qiagen; Hilden, Deutschland)
|
| Growth protocol |
Adipose stromal cells were generated from prmary cultures in two types of medium: standard or EGM2. After 7 days of expansion, cells were harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted with RNeasy Plus Mini kit (Qiagen). cDNA synthesis and amplification were performed on high quality RNA (RQI>9.8) by the Ovation®PicoSL WTA System V2 protocol (NuGEN®).
|
| Label |
biotin
|
| Label protocol |
stained on a GeneAtlas™ System according to the Affymetrix GeneChip® Expression Analysis Technical Manual (P/N 08-0306 Rev. A).
|
| |
|
| Hybridization protocol |
cDNA were fragmented and biotinylated using the Encore™ Biotin Module (NuGEN®) and hybridized (48°C, 20h) to Affymetrix® HuGene1.1STArray Strip Kit (Affymetrix®, Santa Clara, CA, USA)
|
| Scan protocol |
The arrays were scanned using the Affymetrix GeneAtlas imaging station. The scanned images (DAT files) were processed using the AGCC (Affymetrix GeneChip Command Console) software,
|
| Data processing |
The CEL files were imported and manipulated into Partek® Genomics Suite™ software (Partek, Inc. MO, USA).
|
| |
|
| Submission date |
Sep 03, 2019 |
| Last update date |
Sep 04, 2019 |
| Contact name |
Frederic Deschaseaux |
| E-mail(s) |
frederic.deschaseaux@efs.sante.fr
|
| Organization name |
EFS-Occitanie, Inserm U1031
|
| Department |
Research
|
| Lab |
STROMALab
|
| Street address |
4 BIS AVENUE HUBERT CURIEN, BATIMENT INCERE
|
| City |
TOULOUSE |
| State/province |
HAUTE-GARONNE |
| ZIP/Postal code |
31100 |
| Country |
France |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE136778 |
TGFβ1 determines the fate of human adipose-derived stromal cells by restraining their immaturity and white/beige adipocyte potentials |
|
