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Sample GSM4057769 Query DataSets for GSM4057769
Status Public on Sep 04, 2019
Title V07-EGM2
Sample type RNA
 
Source name EGM2 ASCs from subcutaneous adipose tissue
Organism Homo sapiens
Characteristics gender: Female
cell type: adipose-derived stem/stromal cells
growth: EGM2
Treatment protocol Cells were resuspended by trypsination and were lysed with RLT (Qiagen; Hilden, Deutschland)
Growth protocol Adipose stromal cells were generated from prmary cultures in two types of medium: standard or EGM2. After 7 days of expansion, cells were harvested.
Extracted molecule total RNA
Extraction protocol RNA were extracted with RNeasy Plus Mini kit (Qiagen). cDNA synthesis and amplification were performed on high quality RNA (RQI>9.8) by the Ovation®PicoSL WTA System V2 protocol (NuGEN®).
Label biotin
Label protocol stained on a GeneAtlas™ System according to the Affymetrix GeneChip® Expression Analysis Technical Manual (P/N 08-0306 Rev. A).
 
Hybridization protocol cDNA were fragmented and biotinylated using the Encore™ Biotin Module (NuGEN®) and hybridized (48°C, 20h) to Affymetrix® HuGene1.1STArray Strip Kit (Affymetrix®, Santa Clara, CA, USA)
Scan protocol The arrays were scanned using the Affymetrix GeneAtlas imaging station. The scanned images (DAT files) were processed using the AGCC (Affymetrix GeneChip Command Console) software,
Data processing The CEL files were imported and manipulated into Partek® Genomics Suite™ software (Partek, Inc. MO, USA).
 
Submission date Sep 03, 2019
Last update date Sep 04, 2019
Contact name Frederic Deschaseaux
E-mail(s) frederic.deschaseaux@efs.sante.fr
Organization name EFS-Occitanie, Inserm U1031
Department Research
Lab STROMALab
Street address 4 BIS AVENUE HUBERT CURIEN, BATIMENT INCERE
City TOULOUSE
State/province HAUTE-GARONNE
ZIP/Postal code 31100
Country France
 
Platform ID GPL11532
Series (1)
GSE136778 TGFβ1 determines the fate of human adipose-derived stromal cells by restraining their immaturity and white/beige adipocyte potentials

Data table header descriptions
ID_REF
VALUE Unlog data (signal intensity)

Data table
ID_REF VALUE
8000869 6.09568
8047243 32.8611
8049827 141.103
8073548 10.8703
8017039 14.9381
8071234 29.9704
8071259 26.3273
8174692 39.05
8132292 194.316
8114050 51.862
8010161 128.753
8054467 142.184
8054477 57.2114
8095854 397.085
7999206 5.61927
7998117 81.609
7911329 84.2961
7896742 81.609
7911347 81.609
7945342 46.6254

Total number of rows: 33297

Table truncated, full table size 441 Kbytes.




Supplementary file Size Download File type/resource
GSM4057769_V07-EGM2.ga.cel.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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