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Sample GSM405500 Query DataSets for GSM405500
Status Public on May 19, 2009
Title HL-60 + 10 nM TCDD, biological replicate 2
Sample type RNA
Source name HL-60 cells treated for 24h with 10 nM TCDD
Organism Homo sapiens
Characteristics cell line: HL-60 promyelocytic leukemia cell line
leukemia type by fab: M2
agent: TCDD
Treatment protocol HL-60 cells were treated for 24h with the different substances in 5ml tissue flasks
Growth protocol HL-60 cells were cultivated in RPMI medium with 10 % FCS, 1 % penecillin and 1 % streptavidin at 37°C and 5 % CO2
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on human GeneChip U133 2A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Agilent GeneArray Scanner 2500A.
Description n/a
Data processing Cel files were processed using RMA (affy-package, R2.0)
Submission date May 19, 2009
Last update date May 19, 2009
Contact name Charlotte Esser
Phone 00492113389252
Organization name IUF
Department Immunologie
Street address Aufm Hennekamp 50
City Düsseldorf
State/province NRW
ZIP/Postal code 40225
Country Germany
Platform ID GPL571
Series (1)
GSE16160 Expression data of HL-60 leukemia cells after treatment with Curcumin and TCDD

Data table header descriptions
VALUE RMA-processed, log2-transformed signal intensity

Data table
213350_at 8.4
213705_at 4.7
212952_at 10.9
214149_s_at 4.4
212809_at 5.8
213550_s_at 5.7
213826_s_at 6.5
204822_at 5.9
221006_s_at 5.2
221943_x_at 8.3
213789_at 9.3
210053_at 5.5
219406_at 4.2
208952_s_at 5.0
222233_s_at 5.5
60794_f_at 4.9
AFFX-BioB-5_at 7.8
209535_s_at 6.2
205687_at 3.7
216609_at 6.2

Total number of rows: 22277

Table truncated, full table size 325 Kbytes.

Supplementary file Size Download File type/resource
GSM405500.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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