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Sample GSM4039940 Query DataSets for GSM4039940
Status Public on Feb 24, 2020
Title RNA-Seq_HCT116_siLUCRC
Sample type SRA
Source name colorectal cancer
Organism Homo sapiens
Characteristics sirna: siLUCRC
cell line: HCT116
Growth protocol HCT116 cells were cultured in RPMI 1640 medium supplemented with 10% FBS.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol (Takara) following the manufacturer’s protocol followed by DNase I digestion to remove residual DNA.
RNA library preparation was performed by using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (E7420L). Paired-end sequencing was performed with Illumina HiSeq 3000 platform.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Data processing Basecalling was performed using CASAVA version 1.4
Reads were aligned to the hg19 genome assembly using STAR version 2.7.0e.
Genome_build: hg19
Supplementary_files_format_and_content: bigWig
Submission date Aug 20, 2019
Last update date Feb 24, 2020
Contact name Jiancheng Ding
Organization name Xiamen University
Street address School of Pharmaceutical Sciences, Xiamen University Xiang`an South Road
City Xiamen
State/province Fujian
ZIP/Postal code 361102
Country China
Platform ID GPL21290
Series (1)
GSE136040 LncRNA LUCRC regulates colorectal cancer cell proliferation and tumorigenesis by targeting endoplasmic reticulum stress response
BioSample SAMN12606850
SRA SRX6743396

Supplementary file Size Download File type/resource
GSM4039940_RNA-Seq_HCT116_siLUCRCneg.bigWig 149.4 Mb (ftp)(http) BIGWIG
GSM4039940_RNA-Seq_HCT116_siLUCRCpos.bigWig 155.3 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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